Vaccination with Setaria cervi 175 kDa collagenase induces high level of protection against Brugia malayi infection in jirds.
Identifieur interne : 007802 ( Main/Exploration ); précédent : 007801; suivant : 007803Vaccination with Setaria cervi 175 kDa collagenase induces high level of protection against Brugia malayi infection in jirds.
Auteurs : Daya Ram Pokharel [Inde] ; Reeta Rai ; Krithika Nandakumar Kodumudi ; Maryada Venkata Rami Reddy ; Sushma RathaurSource :
- Vaccine [ 0264-410X ] ; 2006.
Descripteurs français
- KwdFr :
- Animaux, Brugia malayi (immunologie), Buffles (parasitologie), Collagenases (immunologie), Femelle, Filariose lymphatique (), Filariose lymphatique (immunologie), Gerbillinae, Lymphocytes auxiliaires Th1 (immunologie), Lymphocytes auxiliaires Th2 (immunologie), Mâle, Setaria (nématode) (enzymologie), Setaria (nématode) (immunologie), Vaccins (immunologie), Vaccins (usage thérapeutique).
- MESH :
- enzymologie : Setaria (nématode).
- immunologie : Brugia malayi, Collagenases, Filariose lymphatique, Lymphocytes auxiliaires Th1, Lymphocytes auxiliaires Th2, Setaria (nématode), Vaccins.
- parasitologie : Buffles.
- usage thérapeutique : Vaccins.
- Animaux, Femelle, Filariose lymphatique, Gerbillinae, Mâle.
English descriptors
- KwdEn :
- Animals, Brugia malayi (immunology), Buffaloes (parasitology), Collagenases (immunology), Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (prevention & control), Female, Gerbillinae, Male, Setaria Nematode (enzymology), Setaria Nematode (immunology), Th1 Cells (immunology), Th2 Cells (immunology), Vaccines (immunology), Vaccines (therapeutic use).
- MESH :
- chemical , immunology : Collagenases, Vaccines.
- enzymology : Setaria Nematode.
- immunology : Brugia malayi, Elephantiasis, Filarial, Setaria Nematode, Th1 Cells, Th2 Cells.
- parasitology : Buffaloes.
- prevention & control : Elephantiasis, Filarial.
- chemical , therapeutic use : Vaccines.
- Animals, Female, Gerbillinae, Male.
Abstract
A zinc containing metalloprotease, 175 kDa collagenase, purified from adult female Setaria cervi showed strong cross-reactivity with sera from putatively immune (PI) individuals (unpublished observation) and induced cytotoxicity to B. malayi L3 larvae and microfilariae by ADCC mechanism [Srivastava Y, Bhandari YP, Reddy MVR, Harinath BC, Rathaur S. An adult 175 kDa collagenase antigen of Setaria cervi in immunoprophylaxis against Brugia malayi. J Helminth 2004;78:347-52]. These preliminary observations suggested the immunoprotective nature of collagenase. To confirm the vaccine potential of this protease, a vaccine trial was conducted in jirds (Meriones unguiculatus) against human filarial parasite B. malayi. The vaccination resulted into a mean protection level of 75.86% and produced high level of protease neutralizing antibodies. Cytokine analysis in immune jirds sera suggested a mixed Th1/Th2 type cellular immune response whereas ELISA, immunoblotting and enzyme antibody inhibition assay revealed the presence of specific anti-collagenase antibodies. Taken together, all these results suggest that S. cervi 175 kDa collagenase could form the basis of an effective molecular vaccine against human lymphatic filariasis.
DOI: 10.1016/j.vaccine.2006.05.103
PubMed: 16870314
Affiliations:
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An adult 175 kDa collagenase antigen of Setaria cervi in immunoprophylaxis against Brugia malayi. J Helminth 2004;78:347-52]. These preliminary observations suggested the immunoprotective nature of collagenase. To confirm the vaccine potential of this protease, a vaccine trial was conducted in jirds (Meriones unguiculatus) against human filarial parasite B. malayi. The vaccination resulted into a mean protection level of 75.86% and produced high level of protease neutralizing antibodies. Cytokine analysis in immune jirds sera suggested a mixed Th1/Th2 type cellular immune response whereas ELISA, immunoblotting and enzyme antibody inhibition assay revealed the presence of specific anti-collagenase antibodies. Taken together, all these results suggest that S. cervi 175 kDa collagenase could form the basis of an effective molecular vaccine against human lymphatic filariasis.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Nandakumar Kodumudi, Krithika" sort="Nandakumar Kodumudi, Krithika" uniqKey="Nandakumar Kodumudi K" first="Krithika" last="Nandakumar Kodumudi">Krithika Nandakumar Kodumudi</name><name sortKey="Rai, Reeta" sort="Rai, Reeta" uniqKey="Rai R" first="Reeta" last="Rai">Reeta Rai</name><name sortKey="Rathaur, Sushma" sort="Rathaur, Sushma" uniqKey="Rathaur S" first="Sushma" last="Rathaur">Sushma Rathaur</name><name sortKey="Reddy, Maryada Venkata Rami" sort="Reddy, Maryada Venkata Rami" uniqKey="Reddy M" first="Maryada Venkata Rami" last="Reddy">Maryada Venkata Rami Reddy</name></noCountry><country name="Inde"><noRegion><name sortKey="Pokharel, Daya Ram" sort="Pokharel, Daya Ram" uniqKey="Pokharel D" first="Daya Ram" last="Pokharel">Daya Ram Pokharel</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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